A nitrogen-fixing bacterial endosymbiont of marine algae is evolving into a nitrogen-fixing organelle, or nitroplast, according to a new study published in Science, thereby expanding a function that was thought to be exclusively carried out by prokaryotic cells to eukaryotes.

Braarudosphaera_bigelowii

Source: Monteiro, F.M., Bach, L.T., Brownlee, C., Bown, P., Rickaby, R.E., Poulton, A.J., Tyrrell, T., Beauf

The dodecahedral coastal coccolithophore Braarudosphaera bigelowii

Eukaryotic cells are remarkably complex and contain various organelles, which are specialized structures within a living cell that have specific biological functions. Two organelles, mitochondria and chloroplasts, play a key role in energy metabolism and photosynthesis, respectively, and likely evolved from the integration of endosymbiotic bacteria to the eukaryotic cell.

Nitrogen fixation

Biological nitrogen (N2) fixation, the conversion of atmospheric N2 gas to biologically available ammonia, is a key metabolic process that maintains the fertility of aquatic and terrestrial systems. N2 fixation in eukaryotes is only known to exist through diverse symbiotic partnerships with prokaryotic microbes capable of N2 fixation. However, the nature of these symbiotic relationships is poorly understood, and, to date, a N2-fixing organelle in eukaryotic cells has yet to be described.

Tyler Coale and colleagues investigated the interactions between Candidatus Atelocyanobacterium thalassa, or UCYN-A, a metabolically streamlined N2-fixing cyanobacterium. which is a known endosymbiont of the unicellular marine algae Braarudosphaera bigelowii.

Through subcellular images taken with soft X-ray tomography to visualize the algae’s cell morphology and division, Coale et al. observed a coordinated cell cycle in which endosymbiotic UCYN-A divided and is split evenly between daughter B. bigelowii cells, which is similar to the way chloroplast and mitochondria organelles are transmitted during cell division.

Imported proteins

Moreover, further proteomic and genomic analyses show that UCYN-A contains many proteins that are imported from the eukaryotic host cell’s nucleus, including those essential for cellular metabolism and control of the cell cycle. According to Coale et al., the findings suggest that UCYN-A has evolved beyond endosymbiosis and functions as an early evolutionary stage N2-fixing organelle. 

“The nitroplast represents a textbook case of a eukaryotic organelle that complements the energy, carbon, and nitrogen needs of the algal host and is another example of how ecology is the theater where evolution takes place,” writes Ramon Massana in a related Perspective.

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