A research group led by Professor Hiroshi Shiigi at the Graduate School of Engineering, Osaka Metropolitan University has developed a technology that can rapidly and accurately determine the number of viable bacteria in food products electrochemically, using tetrazolium salt (MTT), a water-soluble molecule.

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Source: Hiroshi Shiigi, Osaka Metropolitan University

This technology accurately measures viable bacteria in food within 1 hour, as opposed to 2 days, regardless of bacterial species. Unlike existing technologies, this method does not require complicated operations or expensive equipment.

One of the most important assessment indicators for ensuring that food is free from contamination is the number of viable bacteria.

However, conventional measurement methods take up to 2 days to yield results, and these results are only available after the food has been shipped from the factory, leading to potentially fatal consequences. Therefore, it is imperative to have a testing method that speeds up the process of identifying bacterial contamination before shipment.

One hour inspection

The researchers have succeeded in drastically reducing the inspection time from 2 days to about 1 hour, regardless of the bacterial species.

“With this method, we can quickly measure the number of viable bacteria, allowing us to confirm the safety of food products before they leave the factory and to prevent food poisoning,” Professor Shiigi explained.

“This method does not require complicated operations or expensive equipment. Therefore, we will continue to optimize the measurement conditions and expect to see the development of a portable sensor in line with the development of research aimed at practical applications.”

Their results were published in Analytical Chemistry.

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